› 4v11

X-ray diffraction
1.95Å resolution

Structure of Synaptotagmin-1 with SV2A peptide phosphorylated at Thr84

Released:
DOI: 10.2210/pdb4v11/pdb
PDB entry 4v11 coloured by chain, front view.
PDB entry 4v11 coloured by chain, side view.
PDB entry 4v11 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phosphorylation of synaptic vesicle protein 2A at Thr84 by casein kinase 1 family kinases controls the specific retrieval of synaptotagmin-1.
Zhang N, Gordon SL, Fritsch MJ, Esoof N, Campbell DG, Gourlay R, Velupillai S, Macartney T, Peggie M, van Aalten DM, Cousin MA, Alessi DR
J Neurosci 35 2492-507 (2015)
PMID: 25673844

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149304 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Synaptotagmin-1 Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 17.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21579 (Residues: 273-422; Coverage: 36%)
Gene names: SVP65, SYT, SYT1
Sequence domains: C2 domain
Structure domains: C2 domain
Synaptic vesicle glycoprotein 2A Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.16 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q7L0J3 (Residues: 81-90; Coverage: 1%)
Gene names: KIAA0736, PSEC0174, SV2A

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand GOL 1 x GOL
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 46.049Å b: 41.432Å c: 48.014Å
α: 90° β: 96.59° γ: 90°
R-values:
R R work R free
0.153 0.151 0.196
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

16 review citations

Microtubule plus-end tracking proteins in neuronal development.
van de Willige et al. (2016)
15 more

3 mentions without citation

Phosphorylation of synaptic vesicle protein 2A at Thr84 by casein kinase 1 family kinases controls the specific retrieval of synaptotagmin-1.
Zhang et al. (2015)
2 more