› 4uxp

Electron Microscopy
6.3Å resolution

Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins

Released:
DOI: 10.2210/pdb4uxp/pdb
PDB entry 4uxp coloured by chain, front view.
PDB entry 4uxp coloured by chain, side view.
PDB entry 4uxp coloured by chain, top view.
Source organisms:
Primary publication:
Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins.
Atherton J, Farabella I, Yu IM, Rosenfeld SS, Houdusse A, Topf M, Moores CA
OpenAccess logo Elife 3 e03680 (2014)
PMID: 25209998
Related structures: EMD-2766

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + a kinesin associated with a microtubule at position (n) = ADP + phosphate a kinesin associated with a microtubule at position (n+1, toward the plus end)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-160588 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinesin-like protein KIF1A Chain: C
Molecule details ›
Chain: C
Length: 375 amino acids
Theoretical weight: 42.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12756 (Residues: 1-361; Coverage: 21%)
Gene names: ATSV, C2orf20, KIF1A
Sequence domains: Kinesin motor domain

Ligands and Environments

6 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 2 x MG
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.3Å
Relevant EMDB volumes: EMD-2766
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Axonal transport: Driving synaptic function.
Guedes-Dias et al. (2019)
15 more

6 mentions without citation

Intracellular Cargo Transport by Kinesin-3 Motors.
Siddiqui et al. (2017)
5 more