› 4usp

X-ray diffraction
2.25Å resolution

X-ray structure of the dimeric CCL2 lectin in native form

Released:
DOI: 10.2210/pdb4usp/pdb
PDB entry 4usp coloured by chain, front view.
PDB entry 4usp coloured by chain, side view.
PDB entry 4usp coloured by chain, top view.
Source organism: Coprinopsis cinerea
Primary publication:
Dimerization of the fungal defense lectin CCL2 is essential for its toxicity against nematodes.
Bleuler-Martinez S, Stutz K, Sieber R, Collot M, Mallet JM, Hengartner M, Schubert M, Varrot A, Künzler M
Glycobiology 27 486-500 (2017)
PMID: 27980000

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CCL2-like lectin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 16.6 KDa
Source organism: Coprinopsis cinerea
Expression system: Escherichia coli
UniProt:
  • Canonical: B3GA02 (Residues: 2-142; Coverage: 99%)
Gene name: ccl2
Sequence domains: CCL2 lectin-like
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P41212
Unit cell:
a: 59.921Å b: 59.921Å c: 202.907Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.21
Expression system: Escherichia coli

Quick links

Citations

1 review citation

CNL-Clitocybe nebularis Lectin-The Fungal GalNAcβ1-4GlcNAc-Binding Lectin.
Sabotič et al. (2019)
10 other articles

1 mention without citation

Network pharmacology and bioinformatics analyses identify the intersection genes and mechanism of Huang Bai for recurrent aphthous stomatitis.
Tang et al. (2022)