› 4umw

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF A ZINC-TRANSPORTING PIB-TYPE ATPASE IN E2.PI STATE

Released:
DOI: 10.2210/pdb4umw/pdb
PDB entry 4umw coloured by chain, front view.
PDB entry 4umw coloured by chain, side view.
PDB entry 4umw coloured by chain, top view.
Source organism: Shigella sonnei
Primary publication:
Structure and mechanism of Zn2+-transporting P-type ATPases.
Wang K, Sitsel O, Meloni G, Autzen HE, Andersson M, Klymchuk T, Nielsen AM, Rees DC, Nissen P, Gourdon P
Nature 514 518-22 (2014)
PMID: 25132545

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + Zn(2+)(Side 1) = ADP + phosphate + Zn(2+)(Side 2)
ATP + H(2)O + Cd(2+)(Side 1) = ADP + phosphate + Cd(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc/cadmium/lead-transporting P-type ATPase Chain: A
Molecule details ›
Chain: A
Length: 732 amino acids
Theoretical weight: 76.84 KDa
Source organism: Shigella sonnei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3YW59 (Residues: 1-732; Coverage: 100%)
Gene names: SSON_3707, zntA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ALF 1 x ALF
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 77.641Å b: 83.56Å c: 319.818Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Cation Diffusion Facilitator family: Structure and function.
Kolaj-Robin et al. (2015)
13 more

7 mentions without citation

Bacterial Strategies to Maintain Zinc Metallostasis at the Host-Pathogen Interface.
Capdevila et al. (2016)
6 more