PDBe 4u7p

X-ray diffraction
3.82Å resolution

Crystal structure of DNMT3A-DNMT3L complex

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA (cytosine-5)-methyltransferase 3A Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 53.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q9Y6K1 (Residues: 455-912; Coverage: 50%)
Gene name: DNMT3A
Sequence domains:
DNA (cytosine-5)-methyltransferase 3-like Chain: B
Molecule details ›
Chain: B
Length: 209 amino acids
Theoretical weight: 24.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q9UJW3 (Residues: 178-379; Coverage: 52%)
Gene name: DNMT3L
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6322
Unit cell:
a: 252.031Å b: 252.031Å c: 75.336Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.224 0.269
Expression system: Escherichia coli