PDBe 4u5w

X-ray diffraction
1.86Å resolution

Crystal Structure of HIV-1 Nef-SF2 Core Domain in Complex with the Src Family Kinase Hck SH3-SH2 Tandem Regulatory Domains


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C-terminal core protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 149 amino acids
Theoretical weight: 17.59 KDa
Source organism: HIV-1 M:B_ARV2/SF2
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P03407 (Residues: 62-209; Coverage: 71%)
Gene name: nef
Structure domains: Nef Regulatory Factor
Tyrosine-protein kinase HCK Chains: B, D
Molecule details ›
Chains: B, D
Length: 180 amino acids
Theoretical weight: 20.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P08631 (Residues: 72-242; Coverage: 33%)
Gene name: HCK
Sequence domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 56.489Å b: 57.265Å c: 72.111Å
α: 112.28° β: 96.09° γ: 106°
R R work R free
0.17 0.169 0.195
Expression system: Escherichia coli BL21(DE3)