4u4c

X-ray diffraction
2.4Å resolution

The molecular architecture of the TRAMP complex reveals the organization and interplay of its two catalytic activities

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent RNA helicase DOB1 Chain: A
Molecule details ›
Chain: A
Length: 998 amino acids
Theoretical weight: 113.58 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47047 (Residues: 81-1073; Coverage: 93%)
Gene names: DOB1, J1158, MTR4, YJL050W
Sequence domains:
Poly(A) RNA polymerase protein 2; Protein AIR2 Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 12.83 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53632 (Residues: 111-160; Coverage: 9%)
  • Canonical: Q12476 (Residues: 1-62; Coverage: 18%)
Gene names: AIR2, HRC584, O0716, PAP2, TRF4, YDL175C, YOL115W

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 156.184Å b: 166.768Å c: 131.259Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.24
Expression system: Escherichia coli BL21(DE3)