› 4u1p

X-ray diffraction
1.4Å resolution

Human Fyn-SH2 domain in complex with a synthetic high-affinity phospho-peptide

Released:
DOI: 10.2210/pdb4u1p/pdb
PDB entry 4u1p coloured by chain, front view.
PDB entry 4u1p coloured by chain, side view.
PDB entry 4u1p coloured by chain, top view.
Source organisms:
Entry authors: Garcia-Pino A, Huculeci R, Lenaerts T, van Nuland NAJ

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136550 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Fyn Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 14.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06241 (Residues: 148-248; Coverage: 19%)
Gene name: FYN
Sequence domains: SH2 domain
Structure domains: SH2 domain
Middle T antigen Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.47 KDa
Source organism: Alphapolyomavirus mauratus
Expression system: Not provided
UniProt:
  • Canonical: P03079 (Residues: 321-331; Coverage: 3%)

Ligands and Environments

2 bound ligands:
Ligand 15P 1 x 15P
Ligand NA 1 x NA
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P43212
Unit cell:
a: 39.248Å b: 39.248Å c: 145.199Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.188
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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