4tkd

X-ray diffraction
2.01Å resolution

Sulfolobus solfataricus HJC mutants

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction resolvase Hjc Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 141 amino acids
Theoretical weight: 15.8 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7LXU0 (Residues: 1-143; Coverage: 99%)
Gene names: ORF-c21_024, SSO0575, hjc
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 64.601Å b: 61.834Å c: 55.245Å
α: 90° β: 95.74° γ: 90°
R-values:
R R work R free
0.197 0.194 0.246
Expression system: Escherichia coli