4s35

X-ray diffraction
1.55Å resolution

AMPPCP and TMP bound Crystal structure of thymidylate kinase (aq_969) from Aquifex Aeolicus VF5

Released:

Function and Biology Details

Reaction catalysed:
ATP + dTMP = ADP + dTDP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.4 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67099 (Residues: 1-195; Coverage: 100%)
Gene names: aq_969, tmk
Sequence domains: Thymidylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21
Unit cell:
a: 42.479Å b: 62.615Å c: 73.577Å
α: 90° β: 97.97° γ: 90°
R-values:
R R work R free
0.148 0.146 0.174
Expression system: Escherichia coli BL21(DE3)