PDBe 4s02

X-ray diffraction
1.95Å resolution

Biphenylalanine modified threonyl-tRNA synthetase from Pyrococcus abyssi: I11BIF, F42W, Y79A, and F123Y mutant

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). 
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 17.43 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UZ14 (Residues: 1-143; Coverage: 23%)
Gene names: PAB1490, PYRAB13430, thrS
Sequence domains: Archaea-specific editing domain of threonyl-tRNA synthetase

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 52.715Å b: 77.284Å c: 93.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.196
Expression system: Escherichia coli BL21(DE3)