› 4ru2

X-ray diffraction
2.2Å resolution

Crystal structure of a RNA-binding protein 39 (RBM39) in complex with fragment of splicing factor (U2AF) from Mus musculus at 2.20 A resolution

Released:
DOI: 10.2210/pdb4ru2/pdb
PDB entry 4ru2 coloured by chain, front view.
PDB entry 4ru2 coloured by chain, side view.
PDB entry 4ru2 coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Joint Center for Structural Genomics (JCSG), Partnership for T-Cell Biology (TCELL)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150728 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-binding protein 39 Chains: A, C, E, G, I, K, M, O, Q
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q
Length: 114 amino acids
Theoretical weight: 12.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VH51 (Residues: 418-530; Coverage: 21%)
Gene names: Caper, Rbm39, Rnpc2
Structure domains: Alpha-Beta Plaits
Splicing factor U2AF 65 kDa subunit Chains: B, D, F, H, J, L, N, P, R
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R
Length: 29 amino acids
Theoretical weight: 3.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P26369 (Residues: 85-112; Coverage: 6%)
Gene names: U2af2, U2af65

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P32
Unit cell:
a: 127.283Å b: 127.283Å c: 78.864Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.204
Expression system: Escherichia coli

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