X-ray diffraction
1.24Å resolution

Crystal structure of the c-Src-SH3 domain E93V/Q128R mutant in complex with the high affinity peptide APP12

Entry authors: Camara-Artigas A, Bacarizo J

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proto-oncogene tyrosine-protein kinase Src Chains: A, C
Molecule details ›
Chains: A, C
Length: 61 amino acids
Theoretical weight: 6.9 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P00523 (Residues: 85-141; Coverage: 11%)
Gene name: SRC
Sequence domains: SH3 domain
Structure domains: SH3 Domains
APP12 peptide Chains: B, D, E
Molecule details ›
Chains: B, D, E
Length: 13 amino acids
Theoretical weight: 1.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P31
Unit cell:
a: 36.94Å b: 36.94Å c: 83.42Å
α: 90° β: 90° γ: 120°
R R work R free
0.133 0.132 0.158
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided