4rtv

X-ray diffraction
1.37Å resolution

Crystal structure of the Src tyrosine kinase SH3 domain S94A/Q128R mutant in complex with the high affinity synthetic peptide APP12

Released:
Entry authors: Camara-Artigas A, Bacarizo J

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.92 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00523 (Residues: 85-141; Coverage: 11%)
Gene name: SRC
Sequence domains: SH3 domain
Structure domains: SH3 Domains
APP12 peptide Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P3121
Unit cell:
a: 31.559Å b: 31.559Å c: 106.715Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.15 0.178
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided