4rtq

X-ray diffraction
2Å resolution

Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with AdoHcy and a 5-bp non-canonical site (GTTTA )

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA adenine methylase Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 34.33 KDa
Source organism: Escherichia coli str. K-12 substr. MDS42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEE8 (Residues: 1-278; Coverage: 100%)
Gene names: JW3350, b3387, dam
Sequence domains: D12 class N6 adenine-specific DNA methyltransferase
Structure domains:
DNA (5'-D(*AP*CP*TP*TP*AP*AP*AP*CP*TP*TP*AP*A)-3') Chain: F
Molecule details ›
Chain: F
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*AP*AP*GP*TP*TP*TP*AP*AP*G)-3') Chain: G
Molecule details ›
Chain: G
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 44.009Å b: 62.918Å c: 136.642Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.196
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided