4rct

X-ray diffraction
2.25Å resolution

Crystal structure of R-protein of NgoAVII restriction endonuclease

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Restriction endonuclease NgoFVII Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 42.22 KDa
Source organism: Neisseria gonorrhoeae FA 1090
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5F9M9 (Residues: 1-345; Coverage: 100%)
Gene name: NGO_0364
Sequence domains: NgoFVII restriction endonuclease
Structure domains: Endonuclease Chain A

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 75.513Å b: 106.799Å c: 108.235Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.21
Expression system: Escherichia coli