4r8a

X-ray diffraction
3.2Å resolution

Crystal structure of paFAN1 - 5' flap DNA complex

Released:

Function and Biology Details

Reaction catalysed:
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Fanconi-associated nuclease 1 homolog Chains: A, F
Molecule details ›
Chains: A, F
Length: 559 amino acids
Theoretical weight: 64.61 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I2N0 (Residues: 1-559; Coverage: 100%)
Gene names: PA1865, fan1
Sequence domains:
DNA (5'-D(P*AP*CP*CP*AP*GP*AP*CP*AP*CP*AP*CP*AP*TP*TP*C)-3') Chains: B, G
Molecule details ›
Chains: B, G
Length: 15 nucleotides
Theoretical weight: 4.51 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Not provided
DNA (5'-D(P*GP*TP*TP*GP*GP*GP*AP*TP*TP*G)-3') Chains: C, H
Molecule details ›
Chains: C, H
Length: 10 nucleotides
Theoretical weight: 3.13 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Not provided
DNA (5'-D(P*GP*AP*AP*TP*GP*TP*GP*TP*GP*TP*CP*TP*CP*AP*AP*TP*CP*CP*CP*AP*A)-3') Chains: D, I
Molecule details ›
Chains: D, I
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 76.581Å b: 106.663Å c: 142.18Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.197 0.196 0.234
Expression systems:
  • Escherichia coli
  • Not provided