Function and Biology Details
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
- not assigned
Structure analysis Details
Length: 336 amino acids
Theoretical weight: 37.32 KDa
Source organism: Betacoronavirus England 1
Expression system: Escherichia coli BL21(DE3)
- Canonical: K9N638 (Residues: 1481-1811; Coverage: 8%)
Sequence domains: Papain like viral protease