4qrh

X-ray diffraction
1.65Å resolution

Molecular mechanism and evolution of guanylate kinase regulation by (p)ppGpp

Released:

Function and Biology Details

Reaction catalysed:
ATP + GMP = ADP + GDP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 210 amino acids
Theoretical weight: 24.35 KDa
Source organism: Staphylococcus aureus USA300-ISMMS1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0J9X1Z9 (Residues: 4-210; Coverage: 99%)
Gene names: AZ30_05805, gmk
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 69.486Å b: 93.961Å c: 85.259Å
α: 90° β: 111.09° γ: 90°
R-values:
R R work R free
0.202 0.201 0.227
Expression system: Escherichia coli BL21