X-ray diffraction
2.3Å resolution

Crystal structure of dihydrofolate reductase from Yersinia pestis complexed with methotrexate

Source organism: Yersinia pestis CO92
Entry authors: Maltseva N, Kim Y, Makowska-Grzyska M, Mulligan R, Shatsman S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydrofolate reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 167 amino acids
Theoretical weight: 19.26 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli BL21
  • Canonical: A0A384KYY5 (Residues: 1-160; Coverage: 100%)
Gene names: YPO0486, folA
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 178.196Å b: 103.641Å c: 34.288Å
α: 90° β: 93° γ: 90°
R R work R free
0.193 0.191 0.236
Expression system: Escherichia coli BL21