› 4qi2

X-ray diffraction
3Å resolution

X-ray structure of the ROQ domain from murine Roquin-1 in complex with a 23-mer Tnf-CDE RNA

Released:
DOI: 10.2210/pdb4qi2/pdb
PDB entry 4qi2 coloured by chain, front view.
PDB entry 4qi2 coloured by chain, side view.
PDB entry 4qi2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for RNA recognition in roquin-mediated post-transcriptional gene regulation.
Schlundt A, Heinz GA, Janowski R, Geerlof A, Stehle R, Heissmeyer V, Niessing D, Sattler M
Nat Struct Mol Biol 21 671-8 (2014)
PMID: 25026077

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175772 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Roquin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 180 amino acids
Theoretical weight: 20.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4VGL6 (Residues: 147-326; Coverage: 16%)
Gene names: Gm551, Kiaa2025, Rc3h1
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
RNA (5'-R(*AP*CP*AP*UP*GP*UP*UP*UP*UP*CP*UP*GP*UP*GP*AP*AP*AP*AP*CP*GP*GP*AP*G)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 23 nucleotides
Theoretical weight: 7.39 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 56.56Å b: 60.41Å c: 84.37Å
α: 105.68° β: 101.36° γ: 95.72°
R-values:
R R work R free
0.205 0.203 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

19 review citations

RNA-binding proteins in immune regulation: a focus on CCCH zinc finger proteins.
Fu et al. (2017)
18 more

6 mentions without citation

Protein-RNA interactions: structural characteristics and hotspot amino acids.
Krüger et al. (2018)
5 more