PDBe 4qfr

X-ray diffraction
3.34Å resolution

Function and Biology Details

Reactions catalysed:
ATP + [acetyl-CoA carboxylase] = ADP + [acetyl-CoA carboxylase] phosphate
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
5'-AMP-activated protein kinase catalytic subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 503 amino acids
Theoretical weight: 57.78 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54645 (Residues: 11-559; Coverage: 90%)
Gene names: Ampk1, Prkaa1
Sequence domains: Protein kinase domain
5'-AMP-activated protein kinase subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 204 amino acids
Theoretical weight: 23.05 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80386 (Residues: 68-270; Coverage: 75%)
Gene name: Prkab1
Sequence domains:
5'-AMP-activated protein kinase subunit gamma-1 Chain: C
Molecule details ›
Chain: C
Length: 330 amino acids
Theoretical weight: 37.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80385 (Residues: 1-330; Coverage: 100%)
Gene name: Prkag1
Sequence domains: CBS domain

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6122
Unit cell:
a: 124.62Å b: 124.62Å c: 402.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.249
Expression system: Escherichia coli