PDBe 4qf4

X-ray diffraction
1.8Å resolution

Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature

Released:
Source organism: Staphylococcus aureus
Entry authors: Caro JA, Schlessman JL, Heroux A, Garcia-Moreno EB

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.88 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P00644 (Residues: 83-231)
Gene name: nuc

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41
Unit cell:
a: 67.53Å b: 67.53Å c: 62.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.24
Expression system: Escherichia coli BL21(DE3)