PDBe 4q8w

X-ray diffraction
1.14Å resolution

tRNA-Guanine Transglycosylase (TGT) in Complex with 4-[2-({6-Amino-8-oxo-1H,7H,8H-imidazo[4,5-g]quinazolin-2-yl}amino)ethyl]benzoic acid

Entry authors: Neeb M, Heine A, Klebe G

Function and Biology Details

Reaction catalysed:
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.07 KDa
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase
Structure domains: Queuine tRNA-ribosyltransferase-like

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 89.83Å b: 64.68Å c: 70.68Å
α: 90° β: 96.28° γ: 90°
R R work R free
0.135 0.134 0.15
Expression system: Escherichia coli BL21(DE3)