4q7j

X-ray diffraction
2.9Å resolution

Complex structure of viral RNA polymerase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Elongation factor Ts Chains: A, E
Molecule details ›
Chains: A, E
Length: 282 amino acids
Theoretical weight: 30.33 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6P1 (Residues: 2-283; Coverage: 100%)
Gene names: JW0165, b0170, tsf
Sequence domains: Elongation factor TS
Structure domains:
Elongation factor Tu 1 Chains: B, F
Molecule details ›
Chains: B, F
Length: 393 amino acids
Theoretical weight: 43.21 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CE47 (Residues: 2-394; Coverage: 100%)
Gene names: JW3301, b3339, tufA
Sequence domains:
Structure domains:
RNA-directed RNA polymerase subunit beta Chains: C, G
Molecule details ›
Chains: C, G
Length: 594 amino acids
Theoretical weight: 66.31 KDa
Source organism: Escherichia virus Qbeta
Expression system: Escherichia coli
UniProt:
  • Canonical: P14647 (Residues: 2-589; Coverage: 100%)
Sequence domains: RNA replicase, beta-chain
30S ribosomal protein S1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 281 amino acids
Theoretical weight: 31.34 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG67 (Residues: 1-273; Coverage: 49%)
Gene names: JW0894, b0911, rpsA, ssyF
Sequence domains: S1 RNA binding domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 132.19Å b: 150.83Å c: 189.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.255 0.309
Expression system: Escherichia coli