› 4q2a

X-ray diffraction
3.5Å resolution

WNK1: A chloride sensor via autophosphorylation

Released:
DOI: 10.2210/pdb4q2a/pdb
PDB entry 4q2a coloured by chain, front view.
PDB entry 4q2a coloured by chain, side view.
PDB entry 4q2a coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Chloride sensing by WNK1 involves inhibition of autophosphorylation.
Piala AT, Moon TM, Akella R, He H, Cobb MH, Goldsmith EJ
Sci Signal 7 ra41 (2014)
PMID: 24803536

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase WNK1 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 33.12 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9JIH7 (Residues: 194-480; Coverage: 14%)
Gene names: Hsn2, Prkwnk1, Wnk1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BR 1 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 38.005Å b: 58.304Å c: 65.043Å
α: 90° β: 91.35° γ: 90°
R-values:
R R work R free
0.248 0.243 0.297
Expression system: Escherichia coli BL21

Quick links

Citations

75 review citations

Cation-chloride cotransporters in neuronal development, plasticity and disease.
Kaila et al. (2014)
74 more

4 mentions without citation

Nucleotide-binding mechanisms in pseudokinases.
Hammarén et al. (2015)
3 more