X-ray diffraction
1.7Å resolution

Crystal structure of Francisella tularensis HPPK-DHPS in complex with bisubstrate analog HPPK inhibitor J1D


Function and Biology Details

Reaction catalysed:
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 48.31 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli
  • Canonical: Q5NGA7 (Residues: 1-421; Coverage: 100%)
Gene names: FTT_0942c, folK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 42.945Å b: 74.434Å c: 69.758Å
α: 90° β: 93.2° γ: 90°
R R work R free
0.214 0.21 0.259
Expression system: Escherichia coli