4ptf

X-ray diffraction
2.81Å resolution

Ternary crystal structure of yeast DNA polymerase epsilon with template G

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase epsilon catalytic subunit A Chain: A
Molecule details ›
Chain: A
Length: 1194 amino acids
Theoretical weight: 137.46 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21951 (Residues: 1-1187; Coverage: 53%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains:
5'-D(*AP*TP*CP*CP*TP*CP*CP*CP*CP*TP*AP*(DOC))-3' Chain: P
Molecule details ›
Chain: P
Length: 12 nucleotides
Theoretical weight: 3.5 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*TP*AP*AP*GP*GP*TP*AP*GP*GP*GP*GP*AP*GP*GP*AP*T)-3' Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 5.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 147.293Å b: 68.478Å c: 149.079Å
α: 90° β: 109.6° γ: 90°
R-values:
R R work R free
0.186 0.183 0.247
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided