PDBe 4pri

X-ray diffraction
2.4Å resolution

Crystal structure of TK3 TCR-HLA-B*35:08-HPVG complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
MHC class I antigen Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5MK56 (Residues: 25-300; Coverage: 82%)
Gene name: HLA-B
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Epstein-Barr nuclear antigen 1 Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.33 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03211 (Residues: 407-417; Coverage: 2%)
Gene names: BKRF1, EBNA1
T cell receptor alpha constant Chain: D
Molecule details ›
Chain: D
Length: 202 amino acids
Theoretical weight: 22.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: E
Molecule details ›
Chain: E
Length: 240 amino acids
Theoretical weight: 27.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P1
Unit cell:
a: 44.58Å b: 62.38Å c: 100.46Å
α: 98.17° β: 94.59° γ: 109.12°
R-values:
R R work R free
0.213 0.213 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided