› 4pnx

X-ray diffraction
2.41Å resolution

Crystal structure of the complex of lactoperoxidase with bromo methane at 2.41 angstrom resolution

Released:
DOI: 10.2210/pdb4pnx/pdb
PDB entry 4pnx coloured by chain, front view.
PDB entry 4pnx coloured by chain, side view.
PDB entry 4pnx coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of bovine lactoperoxidase with a partially linked heme moiety at 1.98Å resolution.
Singh PK, Sirohi HV, Iqbal N, Tiwari P, Kaur P, Sharma S, Singh TP
Biochim Biophys Acta Proteins Proteom 1865 329-335 (2017)
PMID: 27986533

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactoperoxidase Chain: A
Molecule details ›
Chain: A
Length: 595 amino acids
Theoretical weight: 67.85 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80025 (Residues: 118-712; Coverage: 86%)
Gene name: LPO
Sequence domains: Animal haem peroxidase
Structure domains: Haem peroxidase domain superfamily, animal type

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
4 bound ligands:
Ligand CA 1 x CA
Ligand NAG 4 x NAG
Ligand BMM 1 x BMM
Ligand IOD 13 x IOD
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 53.742Å b: 80.379Å c: 73.177Å
α: 90° β: 103.5° γ: 90°
R-values:
R R work R free
0.223 0.219 0.299

Quick links

Citations

5 citation in other articles

Structures of mouse DUOX1-DUOXA1 provide mechanistic insights into enzyme activation and regulation.
Sun J. (2020)
4 more