PDBe 4pie

X-ray diffraction
1.94Å resolution

Crystal structure of human adenovirus 2 protease a substrate based nitrile inhibitor


Function and Biology Details

Reaction catalysed:
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protease Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.11 KDa
Source organism: Human adenovirus 2
Expression system: Escherichia coli
  • Canonical: P03252 (Residues: 1-204; Coverage: 100%)
Gene name: L3
Sequence domains: Adenovirus endoprotease
Structure domains: Adenoviral Proteinase; Chain A
Protease cofactor Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Human adenovirus 2
Expression system: Not provided
  • Canonical: P03274 (Residues: 240-250; Coverage: 4%)
Gene name: L3

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 41.312Å b: 44.437Å c: 98.921Å
α: 90° β: 90° γ: 90°
R R work R free
0.21 0.207 0.26
Expression systems:
  • Escherichia coli
  • Not provided