PDBe 4peq

X-ray diffraction
2.21Å resolution

Structure of bovine ribonuclease inhibitor complexed with bovine ribonuclease I

Released:

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease pancreatic Chains: A, C
Molecule details ›
Chains: A, C
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain
Ribonuclease/angiogenin inhibitor 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 456 amino acids
Theoretical weight: 48.89 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3SZN8 (Residues: 1-456; Coverage: 100%)
Gene name: RNH1
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I222
Unit cell:
a: 117.792Å b: 123.55Å c: 179.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.226
Expression system: Escherichia coli BL21(DE3)