› 4pah

X-ray diffraction
2Å resolution

HUMAN PHENYLALANINE HYDROXYLASE CATALYTIC DOMAIN DIMER WITH BOUND NOR-ADRENALINE INHIBITOR

Released:
DOI: 10.2210/pdb4pah/pdb
PDB entry 4pah coloured by chain, front view.
PDB entry 4pah coloured by chain, side view.
PDB entry 4pah coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic analysis of the human phenylalanine hydroxylase catalytic domain with bound catechol inhibitors at 2.0 A resolution.
Erlandsen H, Flatmark T, Stevens RC, Hough E
Biochemistry 37 15638-46 (1998)
PMID: 9843368

Function and Biology Details

Reaction catalysed: 
L-phenylalanine + tetrahydrobiopterin + O(2) = L-tyrosine + 4a-hydroxytetrahydrobiopterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine-4-hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 35.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00439 (Residues: 117-424; Coverage: 68%)
Gene name: PAH
Sequence domains: Biopterin-dependent aromatic amino acid hydroxylase
Structure domains: Aromatic amino acid hydroxylase

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand LNR 1 x LNR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2221
Unit cell:
a: 66.78Å b: 108.73Å c: 125.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.208
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural and mechanistic studies on 2-oxoglutarate-dependent oxygenases and related enzymes.
Schofield et al. (1999)
4 more

5 mentions without citation

Tyrosine hydroxylase and regulation of dopamine synthesis.
Daubner et al. (2011)
4 more