› 4p36

X-ray diffraction
1.18Å resolution

Crystal structure of DJ-1 With Zn(II) bound (crystal 2)

Released:
DOI: 10.2210/pdb4p36/pdb
PDB entry 4p36 coloured by chain, front view.
PDB entry 4p36 coloured by chain, side view.
PDB entry 4p36 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Thermodynamic and structural characterization of the specific binding of Zn(II) to human protein DJ-1.
Tashiro S, Caaveiro JM, Wu CX, Hoang QQ, Tsumoto K
Biochemistry 53 2218-20 (2014)
PMID: 24697266

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parkinson disease protein 7 Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 19.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand P15 1 x P15
Ligand ZN 1 x ZN
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P3121
Unit cell:
a: 75.015Å b: 75.015Å c: 75.525Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.119 0.119 0.138
Expression system: Escherichia coli BL21

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Citations

3 review citations

Parkinson's disease: Alterations in iron and redox biology as a key to unlock therapeutic strategies.
Ma et al. (2021)
2 more