PDBe 4owx

X-ray diffraction
2.3Å resolution

Structural basis of SOSS1 in complex with a 12nt ssDNA

Released:
Source organism: Homo sapiens
Primary publication:
Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA.
Cell Rep 6 982-91 (2014)
PMID: 24630995

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Integrator complex subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 56.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q68E01 (Residues: 1-501; Coverage: 48%)
  • Best match: Q68E01-2 (Residues: 1-500)
Gene names: C1orf193, C1orf60, INTS3
Sequence domains: Integrator complex subunit 3
SOSS complex subunit B1 Chain: B
Molecule details ›
Chain: B
Length: 211 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BQ15 (Residues: 1-211; Coverage: 100%)
Gene names: LP3587, NABP2, OBFC2B, SSB1
Sequence domains: OB-fold nucleic acid binding domain
Structure domains: Nucleic acid-binding proteins
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: L
Molecule details ›
Chain: L
Length: 12 nucleotides
Theoretical weight: 3.61 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 98.63Å b: 162.52Å c: 65.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.226
Expression system: Escherichia coli