PDBe 4ot4

X-ray diffraction
1.85Å resolution

X-ray Structure of the Adduct formed between cisplatin and Ribonuclease A

Released:
Source organism: Bos taurus
Primary publication:
Cisplatin binding to proteins: molecular structure of the ribonuclease a adduct.
Inorg Chem 53 3929-31 (2014)
PMID: 24694179

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 99.767Å b: 32.463Å c: 67.575Å
α: 90° β: 93.07° γ: 90°
R-values:
R R work R free
0.192 0.189 0.247
Expression system: Not provided