PDBe 4orz

X-ray diffraction
2Å resolution

HIV-1 Nef protein in complex with single domain antibody sdAb19 and an engineered Hck SH3 domain


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tyrosine-protein kinase HCK Chain: A
Molecule details ›
Chain: A
Length: 67 amino acids
Theoretical weight: 7.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P08631 (Residues: 77-138; Coverage: 12%)
Gene name: HCK
Sequence domains: SH3 domain
Structure domains: SH3 Domains
C-terminal core protein Chain: B
Molecule details ›
Chain: B
Length: 145 amino acids
Theoretical weight: 16.82 KDa
Source organism: HIV-1 M:B_ARV2/SF2
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P03407 (Residues: 45-210; Coverage: 69%)
Gene name: nef
Structure domains: Nef Regulatory Factor
single domain antibody sdAb19 Chain: C
Molecule details ›
Chain: C
Length: 120 amino acids
Theoretical weight: 13.01 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41
Unit cell:
a: 73Å b: 73Å c: 71Å
α: 90° β: 90° γ: 90°
R R work R free
0.204 0.202 0.236
Expression system: Escherichia coli BL21(DE3)