4ogq

X-ray diffraction
2.5Å resolution

Internal Lipid Architecture of the Hetero-Oligomeric Cytochrome b6f Complex

Released:
Primary publication:
Internal Lipid Architecture of the Hetero-Oligomeric Cytochrome b6f Complex.
Structure 22 1008-15 (2014)
PMID: 24931468

Function and Biology Details

Reaction catalysed:
Plastoquinol + 2 oxidized plastocyanin + 2 H(+)(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Cytochrome b6 Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.29 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
UniProt:
  • Canonical: P0A384 (Residues: 1-215; Coverage: 100%)
Gene names: alr3421, petB
Sequence domains: Cytochrome b/b6/petB
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome b6-f complex subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 160 amino acids
Theoretical weight: 17.55 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
UniProt:
  • Canonical: Q93SX1 (Residues: 1-160; Coverage: 100%)
Gene names: alr3422, petD
Sequence domains: Cytochrome b(C-terminal)/b6/petD
Structure domains:
Cytochrome f Chain: C
Molecule details ›
Chain: C
Length: 333 amino acids
Theoretical weight: 35.96 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
UniProt:
  • Canonical: Q93SW9 (Residues: 1-333; Coverage: 100%)
Gene names: all2452, petA
Sequence domains:
Structure domains:
Cytochrome b6-f complex iron-sulfur subunit 1 Chain: D
Molecule details ›
Chain: D
Length: 179 amino acids
Theoretical weight: 19.22 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
UniProt:
  • Canonical: Q93SX0 (Residues: 1-179; Coverage: 100%)
Gene names: all2453, petC1
Sequence domains: Rieske [2Fe-2S] domain
Structure domains:
Cytochrome b6-f complex subunit 6 Chain: E
Molecule details ›
Chain: E
Length: 31 amino acids
Theoretical weight: 3.23 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
UniProt:
  • Canonical: Q8YVQ2 (Residues: 1-31; Coverage: 100%)
Gene names: asl1922, petL
Sequence domains: Cytochrome B6-F complex subunit VI (PetL)
Cytochrome b6-f complex subunit 7 Chain: F
Molecule details ›
Chain: F
Length: 34 amino acids
Theoretical weight: 3.55 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
UniProt:
  • Canonical: P0A3Y1 (Residues: 1-34; Coverage: 100%)
Gene names: asl4754, petM
Sequence domains: PetM family of cytochrome b6f complex subunit 7
Cytochrome b6-f complex subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 37 amino acids
Theoretical weight: 4 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
UniProt:
  • Canonical: P58246 (Residues: 1-37; Coverage: 100%)
Gene names: asr1366, petG
Sequence domains: Cytochrome B6-F complex subunit 5
Cytochrome b6-f complex subunit 8 Chain: H
Molecule details ›
Chain: H
Length: 29 amino acids
Theoretical weight: 3.23 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
UniProt:
  • Canonical: P61048 (Residues: 1-29; Coverage: 100%)
Gene names: asl4263, petN
Sequence domains: PetN

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 159.228Å b: 159.228Å c: 365.879Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.201 0.232