PDBe 4o7v

X-ray diffraction
2.3Å resolution

SAICAR synthetase (Type-2) in complex with ADP and UDP/UMP

Released:
Source organism: Pyrococcus horikoshii OT3
Entry authors: Manjunath K, Jeyakanthan J, Sekar K

Function and Biology Details

Reaction catalysed:
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.48 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57978 (Residues: 1-238; Coverage: 100%)
Gene names: PH0239, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2221
Unit cell:
a: 44.16Å b: 155.12Å c: 78.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.249
Expression system: Escherichia coli