4o3m

X-ray diffraction
2.3Å resolution

Ternary complex of Bloom's syndrome helicase

Released:
Source organism: Homo sapiens
Primary publication:
Structure of human Bloom's syndrome helicase in complex with ADP and duplex DNA.
Acta Crystallogr. D Biol. Crystallogr. 70 1465-75 (2014)
PMID: 24816114

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Bloom syndrome protein Chain: A
Molecule details ›
Chain: A
Length: 659 amino acids
Theoretical weight: 75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P54132 (Residues: 640-1298; Coverage: 47%)
Gene names: BLM, RECQ2, RECQL3
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*GP*TP*CP*GP*AP*GP*AP*TP*CP*CP*AP*AP*G)-3' Chain: P
Molecule details ›
Chain: P
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*TP*TP*GP*GP*AP*TP*CP*TP*CP*GP*AP*CP*GP*CP*TP*CP*TP*CP*CP*CP*TP*TP*A)-3' Chain: T
Molecule details ›
Chain: T
Length: 24 nucleotides
Theoretical weight: 7.25 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 100.93Å b: 164.68Å c: 50.98Å
α: 90° β: 90.14° γ: 90°
R-values:
R R work R free
0.183 0.181 0.215
Expression systems:
  • Escherichia coli
  • Not provided