PDBe 4nv0

X-ray diffraction
1.65Å resolution

Crystal structure of cytosolic 5'-nucleotidase IIIB (cN-IIIB) bound to 7-methylguanosine

Released:

Function and Biology Details

Reactions catalysed:
CMP + H(2)O = cytidine + phosphate
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
7-methylguanosine phosphate-specific 5'-nucleotidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 36.34 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9W197 (Residues: 1-319; Coverage: 100%)
Gene names: CG3362, cN-IIIB
Sequence domains: Pyrimidine 5'-nucleotidase (UMPH-1)
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 46.67Å b: 99.11Å c: 74.12Å
α: 90° β: 90.99° γ: 90°
R-values:
R R work R free
0.161 0.159 0.194
Expression system: Escherichia coli