4nrw

X-ray diffraction
2.84Å resolution

Function and Biology Details

Reactions catalysed:
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Probable formamidopyrimidine-DNA glycosylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 34.51 KDa
Source organism: Acanthamoeba polyphaga mimivirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5UQ00 (Residues: 2-287; Coverage: 100%)
Gene name: MIMI_L315
Sequence domains: Formamidopyrimidine-DNA glycosylase H2TH domain
Structure domains:
5'-D(*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*G)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: Acanthamoeba polyphaga mimivirus
Expression system: Not provided
5'-D(*CP*GP*TP*CP*CP*AP*(3DR)P*GP*TP*CP*TP*AP*C)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 13 nucleotides
Theoretical weight: 3.78 KDa
Source organism: Acanthamoeba polyphaga mimivirus
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 39.401Å b: 121.682Å c: 79.993Å
α: 90° β: 95.68° γ: 90°
R-values:
R R work R free
0.265 0.26 0.314
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided