PDBe 4np5

X-ray diffraction
1.5Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92N at cryogenic temperature

Released:
Source organism: Staphylococcus aureus
Entry authors: Caro JA, Nam S, Schlessman JL, Heroux A, Garcia-Moreno E B

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.12 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P00644 (Residues: 83-231)
Gene name: nuc

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 31.221Å b: 60.453Å c: 38.536Å
α: 90° β: 93.79° γ: 90°
R-values:
R R work R free
0.182 0.18 0.214
Expression system: Escherichia coli BL21(DE3)