PDBe 4nom

X-ray diffraction
2.01Å resolution

Crystal structure of asparaginyl endopeptidase (AEP)/Legumain activated at pH 4.5

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Legumain Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 50.27 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O89017 (Residues: 1-435; Coverage: 100%)
Gene names: Lgmn, Prsc1
Sequence domains: Peptidase C13 family
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 57.952Å b: 167.721Å c: 51.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.204 0.243
Expression system: Spodoptera frugiperda