PDBe 4noj

X-ray diffraction
2.8Å resolution

Crystal structure of the mature form of asparaginyl endopeptidase (AEP)/Legumain activated at pH 3.5


Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Legumain Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.98 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
  • Canonical: O89017 (Residues: 27-289; Coverage: 63%)
Gene names: Lgmn, Prsc1
Sequence domains: Peptidase C13 family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 55.308Å b: 59.579Å c: 96.525Å
α: 90° β: 90° γ: 90°
R R work R free
0.236 0.233 0.294
Expression system: Spodoptera frugiperda