4nod

X-ray diffraction
2.9Å resolution

Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor A, mitochondrial Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 230 amino acids
Theoretical weight: 27.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00059 (Residues: 43-237; Coverage: 79%)
Gene names: TCF6, TCF6L2, TFAM
Sequence domains:
Structure domains: High mobility group box domain
5'-D(*TP*TP*GP*GP*GP*GP*TP*AP*TP*GP*GP*GP*GP*CP*TP*TP*GP*GP*(BRU)P*TP*GP*G)-3' Chains: C, E, I, K
Molecule details ›
Chains: C, E, I, K
Length: 22 nucleotides
Theoretical weight: 7.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
5'-D(*CP*CP*AP*AP*CP*CP*AP*AP*GP*CP*CP*CP*CP*AP*TP*AP*CP*CP*CP*CP*AP*A)-3' Chains: D, F, J, L
Molecule details ›
Chains: D, F, J, L
Length: 22 nucleotides
Theoretical weight: 6.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P1
Unit cell:
a: 56.372Å b: 82.49Å c: 104.222Å
α: 79.86° β: 85.48° γ: 84.53°
R-values:
R R work R free
0.267 0.221 0.267
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli