4nnu

X-ray diffraction
2.81Å resolution

Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor A, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 27.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00059 (Residues: 43-237; Coverage: 79%)
Gene names: TCF6, TCF6L2, TFAM
Sequence domains:
Structure domains: High mobility group box domain
DNA1 Chains: C, E
Molecule details ›
Chains: C, E
Length: 22 nucleotides
Theoretical weight: 6.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
DNA2 Chains: D, F
Molecule details ›
Chains: D, F
Length: 22 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I222
Unit cell:
a: 109.8Å b: 114.38Å c: 144.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.214 0.261
Expression system: Escherichia coli BL21