4nif

X-ray diffraction
2.15Å resolution

Heterodimeric structure of ERK2 and RSK1

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribosomal protein S6 kinase alpha-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 333 amino acids
Theoretical weight: 37.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15418 (Residues: 411-735; Coverage: 44%)
Gene names: MAPKAPK1A, RPS6KA1, RSK1
Sequence domains: Protein kinase domain
Structure domains: Transferase(Phosphotransferase) domain 1
Mitogen-activated protein kinase 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 362 amino acids
Theoretical weight: 41.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28482 (Residues: 1-360; Coverage: 100%)
Gene names: ERK2, MAPK1, PRKM1, PRKM2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 93.49Å b: 87.85Å c: 116.54Å
α: 90° β: 108.22° γ: 90°
R-values:
R R work R free
0.16 0.158 0.208
Expression system: Escherichia coli