X-ray diffraction
2.05Å resolution

Crystal structure of a putative transcriptional regulator from Saccharomonospora viridis in complex with N,N-dimethylmethanamine

Entry authors: Halavaty AS, Filippova EV, Minasov G, Kiryukhina O, Shuvalova L, Endres M, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
HTH tetR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 22.34 KDa
Source organism: Saccharomonospora viridis DSM 43017
Expression system: Escherichia coli BL21(DE3)
  • Canonical: C7MT25 (Residues: 95-284; Coverage: 66%)
Gene name: Svir_16270
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 41.864Å b: 109.24Å c: 73.878Å
α: 90° β: 90° γ: 90°
R R work R free
0.188 0.185 0.259
Expression system: Escherichia coli BL21(DE3)