4nc8

X-ray diffraction
2.17Å resolution

N-terminal domain of delta-subunit of RNA polymerase complexed with nickel ions

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 11.77 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P12464 (Residues: 2-92; Coverage: 53%)
Gene names: BSU37160, rpoE
Sequence domains: HB1, ASXL, restriction endonuclease HTH domain
Structure domains: Arc Repressor Mutant, subunit A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 39.462Å b: 109.636Å c: 82.928Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.224 0.283
Expression system: Escherichia coli